General Cell Based Assays
Cayman Chemical BIlIrubIn asy DMSO 5mL
Bilirubin assay DMSO has been tested and formulated to work exclusively with Cayman’s Total and Direct Bilirubin Colorimetric Assay Kit (Item No. 701720).
Cayman Chemical MItoLIteOrnge FX570 500test
A mitochondrial dye for live and fixed cells; ex/em = 545/575 nm; has been used to label mitochondria in Hep2 cells treated with cisplatin and NAC
Dojindo Molecular Technologies Inc Glutamate Assay Kit-WST (100 tests) is used to determine intracellular levels of Glutamate with easy to adjust dilutions for standard curve and high stability of stock solution 100 tests
Glutamate is not only used for the biosynthesis of proteins and glutathione but also plays an important function as a neurotransmitter. Excess of glutamate is believed to contribute to neurodegenerative diseases such as Alzheimer’s disease. A recent study showed that inhibition of cystine/glutamate transporter (xCT), which takes in cystine and releases glutamate, induces ferroptosis, and current cancer research is exploring xCT as a target. Glutamate Assay Kit-WST enables quantitation of glutamate as a metabolite. This kit has been optimized to quantify glutamate in cell culture supernatants and intracellular glutamate by measuring absorbance in a colorimetric WST reaction. This kit is formatted for 96-well microplates and the assay has a detection limit of 5 μmol/l glutamate. Dojindo has various quantification kits of Intracellular Metabolites. (https://www.dojindo.com/quantification-of-intracellular-metabolites/)
LICOR GAR CellTag™ 700 Stain ICW Kit II
GAR CellTag 700 Stain ICW Kits provide detection reagents for cell-based In-Cell Western™ Assays. This cost-effective normalization method makes quantification of the target protein more precise. CellTag 700 Stain accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number. This kit includes reagents to perform an In-Cell Western assay and CellTag 700 Stain to normalize well-to-well variations in cell number. CellTag 700 accumulates in the nucleus and cytoplasm of permeabilized cells allowing for accurate normalization to cell number. Kit Components include IRDye 800CW Goat anti-Rabbit Secondary Antibody (0.5 mg), Intercept (TBS) Blocking Buffer (1 x 500 mL), CellTag 700 Stain (2 x 10 nmol), and Kit Protocol
Molecular Devices Calcium Assay Kit, QF, Express, 96/384/1536-well
The FLIPR Calcium 6-QF Express Kit from Molecular Devices is a fluorescence-based, homogeneous calcium indicator assay designed for high-throughput intracellular calcium flux screening. The QF formulation supports quench-sensitive assays and multiplexed workflows, and the Express Kit supplies reagent vials sufficient for multiple microplates in 96-, 384-, and 1536-well formats.
- Homogeneous fluorescence-based calcium flux assay for cell-based screening.
- QF formulation for quench-sensitive targets and multiplexing.
- Contains 2 vials of Component A and 2 vials of Component C.
- Assay buffer (Component B) is not included in the Express Kit.
- Sufficient reagent for up to 100 96- or 384-well plates, or 150 1536-well plates.
- Suited for GPCR, ion channel, and other calcium signaling assays in high-throughput screening.
Cayman Chemical Pyruvate asy Detectr 1ea
An internal standard for the quantification of palmitoyl-L-carnitine by GC- or LC-MS
BPS Bioscience Inc CBL-B TR-FRET ASSAY KIT
Human Casitas B-lineage lymphoma proto-oncogene b (CBL-B) is an E3 ubiquitin-protein ligase that functions as a negative regulator of T-cell activation. The CBL-B TR-FRET Assay Kit is designed to measure CBL-B auto-ubiquitination activity in a homogeneous 384 reaction format.
Cayman Chemical NOS ActIvIty asy KIt 1ea
The NOS Activity Assay measures NOS activity by monitoring the conversion of radiolabeled arginine to citrulline. This assay is simple, sensitive, and specific for NOS activity and can be used with both crude and purified enzyme preparations. The kit includes sufficient materials and reagents for 50 total reactions. Radiolabeled arginine and NADPH are not included with the kit.
Molecular Devices Neurotransmitter Uptake Assay Kit, Fluorescence, Live-Cell, 96/384, 50-Plate
The Molecular Devices Neurotransmitter Transporter Uptake Assay Kit is a homogeneous, fluorescence-based live-cell assay for measuring uptake activity of serotonin (SERT), norepinephrine (NET), and dopamine (DAT) transporters. The kit uses a fluorescent substrate and masking dye to enable no-wash, mix-and-read endpoint or kinetic measurements on bottom-read microplate readers.
- Homogeneous, no-wash live-cell assay format.
- Fluorescence-based detection suitable for kinetic or endpoint reads.
- Compatible with 96-well and 384-well black clear-bottom plates.
- Bulk kit contains reagents for 50 plates (5 vials of lyophilized dye/masking mix).
- Reconstituted dye solution stable for limited time; store frozen reagents at -20°C.
Indigo Biosciences Human Insulin Receptor isoform B (INSRb) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Insulin Receptor, isoform B reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against Insulin Receptor, isoform B. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results, and eliminate weeks of cell culture work.
Indigo Biosciences Human Granulocyte-Macrophage Colony-Stimulating Factor Receptor (GM-CSFR, CSF2R) All-inclusive Cell-Based Luciferase Reporter Assay Kit, 96-well assay plate format
Eliminate weeks of cell culture while achieving superior sensitivity with reproducible results from this all-inclusive cell-based luciferase reporter assay. INDIGO’s Human Granulocyte-Macrophage Colony-Stimulating Factor Receptor reporter assay allows you to quickly screen test samples to quantify functional activity, either agonist or antagonist, they may exert against GM-CSFR. Kit includes cryopreserved reporter cells engineered to provide constitutive expression of the receptor, optimized media for use during cell thawing and in diluting test samples, reference agonist, Luciferase Detection Reagent, cell culture-ready assay plate, and detailed assay protocol. INDIGO’s unique Cryomite™ process provides exceptional cell viability post-thaw and INDIGO’s strict QA process means consistent results lot to lot (product quality data available upon request). This kit allows researchers to achieve superior sensitivity, clear, reproducible results